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Volume 45, Number 1
(January - February 2000) |
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Volume 45, Number 1
(January - February 2000) |
Fused Silica Microcolumns, Packing Methods and Their Application in Micro-HPLC
by Bartlomiej Slusarski and Andrzej Stolyhwo
Department of Food Analysis and Quality Control, Faculty of Chemistry, Technical University of Gdansk, 11/12 Narutowicza Str., 80-952 Gdansk, Poland
Key words: liquid chromatography, packed microcolumns, packing the columns
Preparation and utility characteristics of packed microcolumns of 250 mm I.D. filled with silica and chemically modified (C18, NH2) silica are described in this paper. In the case of „long" columns, packed by „dry" technique utilizing carbon dioxide compressed up to 50 MPa, column efficiency equivalent to 34000 theoretical plates with the height of the reduced plate to 2.9 was achieved for Lichrosorb(R) NH2 dp = 10 mm. Optimum flow rate of the mobile phase was determined as equal to ca. 1 ml min-1. The required sample size was about 250 times smaller than for classic (4.6 mm I.D.) HPLC columns.
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