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Volume 45, Number 1
(January - February 2000) |
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Volume 45, Number 1
(January - February 2000) |
Improved Analytical Procedure for the Measurements of Selected Biogenic Polyamines by HPLC Following Dabsyl Derivatisation in Brain Tissue
by Bronislaw K. Glod1, Jan Albrecht2, Wojciech Hilgier2 and Joanna Strosznajder1
1Polish Academy of Sciences, Medical Research Centre, Department of Cellular Signal Transduction
2Polish Academy of Sciences, Medical Research Centre, Department of Neurotoxicology, 5 Pawinskiego Str., 02-106 Warsaw, Poland
Key words: biogenic polyamines, HPLC, dobsyl derivatisation, brain tissue
We present two improvements of a rutine method of analysis of biogenic polyamines after their derivatisation with dabsyl chloride.The use of borate buffer during dabsylation offers much better reproducibility then bicarbonate one. Also trichloracetic acid extraction of polyamines from biological samples was found to be superior to perchloric acid extractin. Our studies eliminate the possibility to determine together biogenic polyamines and amino-acids using common extraction procedure.
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