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Volume 46, Number 6
(November - December 2001) |
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Volume 46, Number 6
(November - December 2001) |
Determination of Morphine and Codeine in Serum and Saliva by Gas Chromatography-Mass Spectrometry
by Ewa Janowska and Wojciech Piekoszewski
Institute of Forensic Research, 9 Westerplatte Street, 31-033 Krakow, Poland
Key words: GC-MS, serum, saliva, morphine, codeine
The GC-MS method of morphine and codeine determination in serum and
saliva was developed and validated. The sample preparation consists of
enzymatic hydrolysis, deproteinisation, solid phase extraction and derivatisation.
As the intrinsic standards D3-morphine
and D3-codeine were used. Detection limit
of morphine and codeine was 4 and 5 ng ml-1;
quatification limit was 14 and 15 ng ml-1
respectively. The range of quantification was 20-1000 ng ml-1
and recovery ranged 50-60%. For checking the applicability of the elaborated
method, morphine and codeine concentrations were measured in serum and
saliva samples taken from 10 addicts. The method allowed to measure morphine
and codeine in saliva and may be used for monitoring taking home made heroin
and detoxification process.
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