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Volume 46, Number 6
(November - December 2001) |
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Volume 46, Number 6
(November - December 2001) |
A Simple and Fast Method for the Determination of Ranitidine in Human Plasma by HPLC-DAD
by Victor David1, Mihaela Ionescu2, Florentin Tache1 and Andrei Medvedovici1
1University
of Bucharest, Faculty of Chemistry, Department of Analytical Chemistry,
Sos. Panduri, no.90, Sect. 5, Bucharest, Romania
2Biopharmacy
and Pharmacology Res., Bucharest, Romania
Key words: ranitidine, human plasma, liquid chromatography
A simple and fast method for the determination of ranitidine in plasma
samples is described.Thie method is based on plasma deproteinization
in the presence of trichloroacetic acid, and determination by HPLC-DAD,
on a XDB C18 column, at 320 nm. Elution
was isocratic, using a mobile phase containing 60% phosphate buffer 20
mmol l-1 (pH 7.3) and 40% methanol, with
flow rate of 1 ml min-1, at 40 oC.
Applied to a bioequivalence study, this method reaches a limit of determination
of 15 ng ml-1 ranitidine in plasma samles,
for an injection volume of 50 ml.
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