experience

Research Experience

 

  • 2012-now: Senior NMR Project Manager, C4X Discovery Ltd., Manchester, UK
  • determined 4D-structures of molecules in context of drug design

 

  • 2011-2012: Research Associate, Laboratory of Molecular Interactions, Faculty of Chemistry, Warsaw University, Warsaw, Poland

Supervisor: Professor Wiktor Kozminski

  • determined Bacillus subtilis Lipase A structure using 4D non-uniformly sampled NMR spectra

 

  • 2008-2010: Senior Scientist, Max-Planck-Institut fur Kohlenforschung, Mulheim an der Ruhr, Germany

Supervisor: Professor Manfred T. Reetz

  • studied Bacillus subtilis Lipase A thermostability using NMR spectroscopy, X-ray crystallography, circular dichroism and enzymatic assays

 

  • 2005-2008: Research Associate, Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA, USA

Supervisor: Professor Kurt Wuthrich

  • determined protein structures with NMR spectroscopy
  • analyzed protein data bases using bioinformatics

 

  • 2004-2005: Research Assistant, Laboratory of Peptides, Faculty of Chemistry, Warsaw University, Warsaw, Poland
  • studied mechanism of action of tyrosine phenol-lyase

 

  • 2004-2005: Volunteer Researcher, Department of Biophysics, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland

Supervisor: Dr. Igor Zhukov

  • interpreted NMR spectra of s100A1 and e1 (C-terminal domain) proteins

 

  • 1999-2004: Ph.D. Student, Laboratory of Peptides, Faculty of Chemistry, Warsaw University, Warsaw, Poland

Supervisor: Professor Marianna Kanska

  • designed, developed and optimized new routes of syntheses of stereospecifically labeled aromatic amino acids
  • elaborated methods of determination of kinetic isotope effects, and used them in the studies on L-tyrosine phenol lyase
  • discovered and proved details of the mechanism of action of L-tyrosine phenol lyase

 

  • 1999-2002: Volunteer Researcher, Department of Genetics, Faculty of Biology, Warsaw University, Warsaw, Poland

Supervisor: Dr. Piotr Borsuk

  • studied polymorphism of mammals’ insulin C-peptide

 

  • 2001: Socrates-Erasmus Program Student, Department of Organic Chemistry, Radboud University of Nijmegen, Nijmegen, The Netherlands.

Supervisors: Professor Floris P. J. T. Rutjes, Christien Schortinghuis

  • synthesized substituted prochiral malonodinitriles, and analysed products of their enzymatic hydrolyses
  • synthesized 5-13C labeled 3-hydroxy-5-cyanobutyramide, and studied mechanism of its enzymatic racemisation using 13C-NMR

 

  • 1999-2000: Volunteer Researcher, Department of Biophysics, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland

Supervisor: Dr. Aleksandra Wyslouch-Cieszynska

  • synthesized peptides on solid phase support: phosphorylated and non-phosphorylated fragment of human eIF4E, Hodges helix derivatives, marinostatin D

 

  • 1997-1999: M.Sc. Student, Laboratory of Peptides, Faculty of Chemistry, Warsaw University, Warsaw, Poland

Supervisor: Professor Marianna Kanska

  • synthesized 14C labeled L-phenylalanines, and used them for determination of kinetic isotope effects in the reaction catalysed by L-phenylalanine ammonia lyase

 

  • 1997-1999: Volunteer Researcher, Department of Molecular Biology, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Poland

Supervisor: Professor Tadeusz Kulikowski

  • synthesized 5-methyluridine, its 5’-monophosphate, and their derivatives

 

research grants

 

  • 2009-2010: EU-NMR initiative of European Commission: NMR Studies of Lipase Thermostability

 

  • 2002-2004: State Committee for Scientific Research Ph.D. Grant 4 T09A 039 23: Syntheses of isotopomers of L-tyrosine and their use in studies of the mechanism of action of beta-tyrosinase. Execution evaluated as excellent.