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Educational and
professional goals:
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Understanding of specificity of interactions of metal ions with biomolecules. Knowledge of construction, operating principles, and applicability of enzymatic and DNA biosensors.
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Course description:
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Detailed analysis of structural and catalytic role of metal ions in proteins, enzymes and DNA. Relation between properties of metal ions and complexing abilities of biomolecules. Biosensors: definition, classification, basic operating principles, history of commercial biosensors with a focus on amperometric biosensors. First, second and third generation of amperometric biosensors: redox reactions; oxidation and reduction of hydrogen peroxide; use of redox active mediators; electroanalytical methods of detection; commercially available glucose biosensors; application of glucose biosensors in food and drink industries; detection of alcohols and aldehydes; detection of toxic metal ions. Biofuel cells: reduction of oxygen; use of laccase and other metal containing enzymes. Interactions of DNA with alkali and transition metal ions. DNA biosensors: principles of operation and applicability; electroanalytical methods of detection; use of complexes of transition metal ions as mediators of the oxidation of DNA intercalators.
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