Under construction

Under construction

My studies are focused on the efficiency of the transport of selected anticancer drugs (doxorubicin and daunomycin) through model biological membranes. Simple models of membranes consist of phospholipid mono- and bilayers and are prepared using Langmuir technique. Due to the electrochemical activity of the selected anticancer drugs their transport can be directly followed by means of electrochemical techniques (cyclic voltammetry, square wave voltammetry, chronocoulometry) and visualized by microscopic techniques. Drug nanocarriers such as gold nanoparticles and carbon nanotubes help improve drug bioavailability and increase the accumulation of a drug only in the infected areas of the body. Therefore, it is very important to determine the influence of potential drug carriers on the model biological membranes. Changes in the structure and organization of the membranes caused by the presence of the investigated nanostructures allow one to describe the mechanism of drug transport mediated by carriers, to identify the nature of the interactions between drug nanocarriers and the components of model membranes as well as to determine the conditions facilitating or inhibiting the transport. Different techniques including electrochemical, microscopic (AFM, STM, TEM) and spectroscopic (PMIRRAS) methods are used to characterize structural changes of model membranes caused by the presence of drug nanocarriers.

Tailored lipidic mesophases we apply as the novel matrices for biosensing, biofuel cells and in drug delivery systems. Such lipidic based liquid-crystalline material is non-toxic and biodegradable which is important in case of applications of the devices in the biological environment. These materials exhibit high phase stability, optical transparency and chemical permeability. They enable incorporation of various proteins, as well as soluble and membrane enzymes, which retain their activity and structure in the host lipidic environment. From the electrochemical point of view it is important that amphiphilic enzyme molecule remains attached to the lipid bilayer, while water channels allows the enzymatic substrates and products to diffuse freely through the system. The aim of our work is to design new lipid molecules as additives to form cubic phases with designed functionalities. Such structures will be used as the matrix to immobilize biocatalysts and selected drugs. The structure and dynamics of these materials will be characterized, and they will be implemented in biomolecular devices. By using appropriately tailored new molecules we can improve mass transport in the phase, its stability and mechanical properties. Even more importantly, we can finally employ a wide range of membrane proteins to work as biocatalysts of the mediatorless processes required in the biofuel cells.

In my research I investigate various nanomaterials: nanotubes, carbon nanoparticles, fullerenes, boron-doped diamond, metal nanoparticles, composite nanoparticles for improving the properties of electrodes to be used in the enzymatic biofuel cells. Biofuel cells are alternative energy sources and their potential applications are to power small devices such as sensors, meters, toys or medical instruments. They can be used as a powering unit of self-powered biosensors and drug dispensers for medicine and environmental protection. As part of the development of new drug dosing devices, I study the interactions of drugs with various nanomaterials, e.g. gold nanoparticles, potentially useful in anti-cancer therapies.

I am particularly interested in the:

• construction of bioelectrochemical, enzyme-based oxygen detection systems,
• designing new bioelectrochemical systems containing enzymes for applications in biofuel cells and sensors,
• design and use of self-powered measuring systems, such as a potentiostat with a sensor, for monitoring chemical compounds in the environment or living organisms,
• application of enzymes in fuel cell technology, design of hybrid systems, enzymatic fuel cells and supercapacitors,
• the use of gold nanoparticles as drug carriers,
• studies of the catalytic activity of enzymes adsorbed on particles,
• design and application of diffusion electrodes for gases,
• studies of electron transfer mechanisms,
• synthesis and study of the properties of metal nanoparticles, mainly gold and platinum nanoparticles used as catalysts and in the construction of fuel cells and sensors.

2020

E.U. Stolarczyk, A. Leś, M. Łaszcz, M. Kubiszewski, W. Strzempek, E. Menaszek, M. Fusaro, K. Sidoryk, K. Stolarczyk, "The ligand exchange of citrates to thioabiraterone on gold nanoparticles for prostate cancer therapy", International Journal of Pharmaceutics (2020) 583 (2020) 119319, IF (2019) = 4.213

K. Stolarczyk, J. Rogalski, R. Bilewicz, "NAD(P)-dependent glucose dehydrogenase for bioelectrodes and biofuel cells", Bioelectrochemistry 135 (2020) 107574, IF (2019) = 4.474

M. Kizling, M. Dzwonek, A. Nowak, Ł. Tymecki, K. Stolarczyk, A. Więckowska, R. Bilewicz, "Multi-substrate biofuel cell utilizing glucose, fructose and sucrose as the anode fuels", Nanomaterials. 10(8) (2020) 1534, IF (2019) = 4,324

2018

B. Olszewski, K. Stolarczyk, "Laccase catalyzed reduction of oxygen at electrodes modified by carbon nanotubes with adsorbed promazine or acetosyringone", Catalysts 8 (2018) 414 , IF (2018) = 3,465

E.U. Stolarczyk, M. Łaszcz, A. Leś, M. Kubiszewski, K. Kuziak, K. Sidoryk, K. Stolarczyk, "Preparation and characterization of abiraterone-functionalized gold nanoparticles - physicochemical studies and molecular modelling", Nanomaterials 8 (2018) 641, IF (2018) = 3,504

E.U. Stolarczyk, K. Sidoryk, M. Cybulski, M. Kubiszewski, K. Stolarczyk, "Design of therapeutic self-assembled monolayers of thiolated abiraterone", Nanomaterials 8 (2018) 1018, IF (2018) = 3,504

2017

M Kizling, M. Dzwonek, B. Olszewski, P. Bącal, Ł. Tymecki, A. Więckowska, K. Stolarczyk, R. Bilewicz, "Reticulated vitreous carbon as a scaffold for enzymatic fuel cell designing", Biosensors and Bioelectronics 95 (2017) 1 - 7, IF (2017) = 8,173

E.U. Stolarczyk, K. Stolarczyk, M. Łaszcz, M. Kubiszewski, W. Maruszak, W. Olejarz, D. Bryk, "Synthesis and characterization of genistein conjugated with gold nanoparticles and the study of their cytotoxic properties", European Journal of Pharmaceutical Sciences 96 (2017) 176 - 185, IF (2017) = 3,466

W. Maruszak, E.U. Stolarczyk, K. Stolarczyk, "CE method for the in-process control of the synthesis of active substances conjugated with gold nanoparticles", Journal of Pharmaceutical and Biomedical Analysis 141 (2017) 52 - 58, IF (2017) = 2,831

E.U. Stolarczyk, K. Stolarczyk, M. Łaszcz, M. Kubiszewski, A. Leś, O. Michalak, "Pemetrexed conjugated with gold nanoparticles - synthesis, characterization and a study of noncovalent interactions", European Journal of Pharmaceutical Sciences 109 (2017) 13 - 20 IF (2017) = 3,466

2016

M. Kizling, K. Stolarczyk, P. Tammela, Z. Wang, L. Nyholm, J. Golimowski, R. Bilewicz, "Bioelectrodes based on pseudocapacitive cellulose/polypyrrole composite improve performance of biofuel cell", Bioelectrochemistry 112 (2016) 184 - 190, IF (2016) = 3,789

M. Kizling, P. Biedul, D. Zabost, K. Stolarczyk, R. Bilewicz, "Application of hydroxyethyl methacrylate and ethylene glycol methacrylate phosphate copolymer as hydrogel electrolyte in enzymatic fuel cell", Electroanalysis 28 (2016) 2444 - 2451, IF (2016) = 2,851

2015

M. Kizling, K. Stolarczyk, J. Sim Sin Kiat, P. Tammela, Z. Wang, L. Nyholm, R. Bilewicz, "Pseudocapacitive polypyrrole-nanocellulose composite for sugar-air enzymatic fuel cells", Electrochemistry Communications 50 (2015) 55 - 59, IF (2015) = 4,660

M. Kizling, S. Dramińska, K. Stolarczyk, P. Tammela, Z. Wang, L. Nyholm, R. Bilewicz, "Biosupercapacitors for powering oxygen sensing devices", Bioelectrochemistry 106 (2015) 34 - 40, IF (2015) = 3,789

D. Majdecka, S. Dramińska, K. Stolarczyk, M. Kizling, P. Krysiński, J. Golimowski, J.F. Biernat, R. Bilewicz, "Sandwich biobattery with enzymatic cathode and zinc anode integrated with sensor", Journal of The Electrochemical Society 162 (6) (2015) F555 - F559, IF (2015) = 3,662

Mazur, B. Rola, K. Stolarczyk, E. Nazaruk, R. Bilewicz, J. Rogalski, S. Ohga, "The large scale production of Cerrena unicolor laccase on waste", Agricultural Based Media Journal - Faculty of Agriculture Kyushu University 60 (2) (2015) 297 - 302, IF (2015) = 0,351

E.U. Stolarczyk, K. Stolarczyk, M. Kubiszewski, "Właściwości i zastosowanie nanocząstek złota, jako selektywnych nanonośników leków w transporcie antynowotworowych substancji czynnych", Farmacja Polska 71(2) (2015) 111 - 118

2014

K. Stolarczyk, M. Kizling, D. Majdecka, K. Żelechowska, J.F. Biernat, J. Rogalski, R. Bilewicz, "Biobatteries and biofuel cells with biphenylated carbon nanotubes", Journal of Power Sources 249 (2014) 263 - 269, IF (2014) = 6,945

D. Majdecka, S. Dramińska, K. Stolarczyk, M. Kizling, P. Krysiński, J. Golimowski, R. Bilewicz, "Sandwich biobattery with enzymatic cathode and zinc anode for powering sensors", ECS Transaction 61 (2014) 1 - 7

M. Skunik-Nuckowska, K. Grzejszczyk, K. Stolarczyk, R. Bilewicz, P.J. Kulesza, "Integration of supercapacitors with enzymatic biobatteries towards more effective pulse-powered use in small-scale energy harvesting devices", Journal of Applied Electrochemistry 44 (2014) 497 - 507, IF (2014) = 2,262

2013

K. Żelechowska, K. Stolarczyk, D. Łyp, J. Rogalski, K.P. Roberts, R. Bilewicz, J.F. Biernat, "Aryl and N-arylamide carbon nanotubes for electrical coupling of laccase to electrodes in biofuel cells and biobatteries", Biocybernetics and Biomedical Engineering 33 (2013) 235 - 245, IF (2013) = 1,374

K. Żelechowska, K. Stolarczyk, D. Łyp, J.F. Biernat, J. Rogalski, R. Bilewicz, "Materials for mediator-free electron transfer in the enzymatic electrodes of biobatteries and biofuel cells", ECS Transactions 45 (11) (2013) 1 - 8

2012

K. Stolarczyk, M. Sepełowska, D. Łyp, K. Żelechowska, J.F. Biernat, J. Rogalski, K.D. Farmer, K.N. Roberts, R. Bilewicz, "Hybrid biobattery based on arylated carbon nanotubes and laccase", Bioelectrochemistry 87 (2012) 154 - 163, IF (2012) = 3,947

K. Stolarczyk, D. Łyp, K. Żelechowska, J.F. Biernat, J. Rogalski, R. Bilewicz, "Arylated carbon nanotubes for biobatteries and biofuel cells", Electrochimica Acta 79 (2012) 74 - 81, IF (2012) = 5,116

2011

R. Bilewicz, E. Nazaruk, K. Żelechowska, J.F. Biernat, K. Stolarczyk, K.P. Roberts, G. Ginalska, J. Rogalski, "Carbon nanotubes chemically derivatized with redox systems as mediators for biofuel cell applications", Biocybernetics and Biomedical Engineering 31 (2011) 17 - 30, IF (2011) = 1,374

2010

K. Sadowska, K. Stolarczyk, J.F. Biernat, K.P. Roberts, J. Rogalski, R. Bilewicz, "Derivatization of single-walled carbon nanotubes with redox mediator for biocatalytic oxygen electrodes", Bioelectrochemisty 80 (2010) 73 - 80, IF (2010) = 3,789

2009

R. Bilewicz, K. Stolarczyk, K. Sadowska, J. Rogalski, J.F. Biernat, "Carbon nanotubes derivatized with mediators for laccase catalyzed oxygen reduction", ECS Transactions 19 (2009) 27 - 36

2008

P6. K. Sadowska, E. Jabłonowska, K. Stolarczyk, R. Wiser, R. Bilewicz, K.P. Roberts, J.F. Biernat, "Chemically modified carbon nanotubes: synthesis and implementation", Polish Journal of Chemistry 82 (2008) 1309 - 1313, IF (2008) = 0,518

K. Stolarczyk, E. Nazaruk, J. Rogalski, R. Bilewicz, "Nanostructured carbon electrodes for laccase catalyzed oxygen reduction without added mediators", Electrochimica Acta 53 (2008) 3983 - 3990, IF (2008) = 5,116

2007

K. Stolarczyk, E. Nazaruk, J. Rogalski, R. Bilewicz, "Mediatorless catalytic oxygen reduction at boron-doped diamond electrodes", Electrochemistry Communications 9 (2007) 115 - 118, IF (2007) = 4,660

E.U. Stolarczyk, K. Stolarczyk, "Perspektywy nanotechnologii w medycynie i farmacji i jej wpływ na przemysł farmaceutyczny. Perspectives of nanotechnology in medicine and pharmacy and its influence on Pharmaceutical Industry", Przemysł Chemiczny 86/7 (2007) 797 - 799, IF (2007) = 0,399

2006

K. Stolarczyk, R. Bilewicz, "Electron transport through alkanethiolate films decorated with monolayer protected gold clusters", Electrochimica Acta 51 (2006) 2358 - 2365, IF (2006) = 5,116

2004

K. Stolarczyk, R. Bilewicz, A. Skwierawska, J.F. Biernat, "Functionalization of electrode surfaces with monolayers of azocompounds and gold clusters", Journal of Inclusion Phenomena 49 (2004) 173 - 179, IF (2004) = 1,316

K. Stolarczyk, B. Pałys, R. Bilewicz, "Catalytic properties of 4-hydroxythiophenol protected gold nanoclusters supported on gold electrodes", Journal Electroanalytical Chemistry 564 (2004) 93 - 98, IF (2004) = 3,235

K. Stolarczyk, R. Bilewicz*, "Catalytic oxidation of ascorbic acid on 2D and 3D monolayers of 4-hydroxythiophenol", Electroanalysis 16 (2004) 1609 - 1615, IF (2004) = 2,851

2003

K. Stolarczyk, R. Bilewicz, L. Siegfried, T. Kaden, "The dual role of self assembled monolayers of tetraazamacrocyclic copper (II) complex in ascorbate oxidation catalysis", Inorganica Chimica Acta 348 (2003) 129 - 134, IF (2003) = 2,264

Under construction

Investigation of the interactions between statins and model cell membranes at the air-water interface using Langmuir technique. Design and characterization of statin delivery systems.