_____________________Fluorimeter_____________________

Fluorolog-3 (Horiba Jobin-Yvon) with TCSPC option (Time-Correlated Single Photon Counting) is a highly sensitive fluorescence spectrophotometer enabling measurements of steady-state spectra and time-resolved measurements at picosecond resolution. The instrument operates within a wide spectral range from UV to NIR enabling studies of liquid and solid samples. In the steady-state mode, the sample is excited by the xenon lamp, while the luminescent or laser diodes are used as sources in time-resolved measurements. The spectrometer is equipped with two detectors: PMT (range 250 nm - 800 nm) and liquid nitrogen-cooled CCD camera (range 300 - 900 nm). A total internal reflection fluorescence accessory is also available. The instrument is equipped with polarizers which allows measurements of steady state and time-resolved anisotropy.

Applications:

• Registering of emission and excitation spectra of molecules or nanoparticles
• Measuring fluorescence lifetimes for times up to 100 ps
• Determination of rotational dynamics of the molecules in solutions, micellar systems, etc. (anisotropy measurements)

AFM1

Fluorimeter set-up